Unfortunately, peripheral blood cell counts are not available for our subjects. However, a search of the literature disclosed no association between CWP and neutropenia. Both SACE and serum lysozyme levels have been previously shown to be elevated in association with silicosis.2* The combination found in coal workers in the present study of the elevation of […]
In a previous investigation, alveolar macrophages collected by bronchoal-veolar lavage from subjects exposed to inorganic dusts were demonstrated to be activated. Endothelial cells are also a rich source of angiotensin-converting enzyme. In contrast, they are not a source of lysozyme. Thus, stimulation of endothelial cells in association with recent exposure to coal mine dust, causing […]
Exposure to coal dust does not result in a granulomatous response and thus the source of SACE is probably not granuloma-associated epithelioid cells as is the case for sarcoidosis. Furthermore, cellular changes specifically associated with the formation of the coal macule, the characteristic pathologic lesion of CWP, must not have been the source of elevated […]
The miners with exposure within three years (13.3 ± 1.3 mg/L) or not (12.9 ± 1.5 mg/L) or those with CWP (14.8 ±1.6 mg/L) or without CWP (11.9 ±1.1 mg/L) all had serum lysozyme concentrations less than the normal controls (p<0.05, all comparisons). The subjects with active sarcoidosis had elevated levels of both SACE and […]
In contrast, the subjects with sarcoidosis were younger (39.8 ±3.1 years) and included only six (30 percent) male subjects and five (25 percent) smokers. For the miners as a group, SACE (49.1 ±2.7 U/ml) did not differ from the normal controls (43.8 ± 1.5 U/ ml). However, within the miners there was a negative correlation […]
The three-year cutoff segregated the two clusters of data well. Data were expressed as mean ± SEM. Results from different groups were compared using analysis of variance (ANOVA) with Fishers LSD test. The correlation between SACE activity and smoking history and time since exposure was made using Spearmans rank correlation. Prevalences of smoking in subject […]
Following phlebotomy, serum was separated and stored at — 20°C until analyzed. SACE activity was determined by a reference laboratory by measuring the release of the dipeptides benzoyl glycine and histidyl leucine from the synthetic substrate benzoyl-glycyl-histidyl-leucine. Lysozyme activity was determined locally by the lysoplate technique.
The subjects with sarcoidosis were included as positive controls for the measurement of SACE and lysozyme. The diagnosis of sarcoidosis was based on the presence of non-caseating granulomas found by tissue biopsy in the setting of typical clinical features, ie} lymphadenopathy, pulmonary involvement, cutaneous plaques, or laboratory findings, ie, hypergammaglobulinemia, hypercalcemia, or hypercalciuria.
The results suggest that underground coal mining, but not CWP, is associated with elevations in SACE and that after removal from the exposure to mixed coal mine dusts, the levels of SACE normalize. Furthermore, serum lysozyme was not elevated, suggesting that the response of alveolar macrophages to the inhalation of coal dust differs from the […]
Measurement of serum angiotensin-converting enzyme (SACE) has been clinically applied as a marker of inflammatory activity in sarcoidosis. It has also been noted to be elevated in silicosis,2″ presumably in association with disease activity and in asbes-tosis. Alveolar macrophages contain angiotensin-converting enzyme and may be a source of SACE activity common to the pneumoconiosis. Furthermore, […]