27 Oct

Interactions between Cigarette Smoking and the Natural History of Idiopathic Pulmonary Fibrosis: Methods (Part 2)

Bronchoalveolar Lavage
Bronchoalveolar lavage was performed as previously described.
Briefly, after wedging a fiberoptic bronchoscope in a subsegment of the lingula, 3 aliquots of 50 ml of sterile 0.9 percent saline solution, preheated to 37°C, were instilled and rapidly recovered by gentle aspiration. The fluid was collected in a silicone glass container and transported to the laboratory where the cells were immediately counted. Slides were prepared by cytocentrifugation (Shandon), stained by the May Grunwald Ciemsa method and a differential cell count was performed. ventolin inhalers
Assessment of Prednisolone Responsiveness
All patients received a standard prednisolone regimen: the initial dose was 1 mg/kg/day for four weeks, followed by 0.75 mg/kg/day for the next four weeks and then the dosage was tapered gradually based on clinical response. All patients were reevaluated by PFT between four to eight weeks after therapy began. Responsiveness was assessed by changes of TLC, Pa02 at rest and Dsb when compared with initial values (ATLC %, APa02 mm Hg, A Dsb %).
Data Analysis
Data are expressed as mean ± standard error of the mean. Comparisons between patient groups were made using the Mann-Whitney or x2 tests when appropriate, and Spearmans rank correlation coefficient (rj was used to calculate relationships between BAL cells and PFT changes or cigarettes smoked per day. Survival was assessed by the Kaplan-Meier method.

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