Archive for the 'Protein' Category

26 Jan

Isoforms of the Mitochondrial Capsule Protein: DISCUSSION

Protein tyrosine phosphorylation is one of the important biochemical mechanisms regulating sperm capacitation. In the present study, we found the time-dependent increase in protein tyrosine phosphorylation of hamster spermatozoa in media that supports capacitation. It has been proposed that the tyrosine phosphorylation of an 80-kDa hamster sperm protein, localized to the principal piece of the […]

24 Jan

Isoforms of the Mitochondrial Capsule Protein: RESULTS

Pattern of Capacitation-Dependent Tyrosine-Phosphorylated Polypeptides of Cauda Epididymal Spermatozoa Western blots of capacitated sperm lysates stained with anti-pY20 revealed a spectrum of tyrosine phosphorylated polypeptides of Mr 19 000-99 000 (Fig. 1A, lane 2); no bands were detected in the lysates of noncapacitated spermatozoa (Fig. 1A, lane 1). When the anti-pY20 was preincubated with o-phospho-DL-tyrosine […]

22 Jan

Isoforms of the Mitochondrial Capsule Protein: MATERIALS AND METHODS

Animals Care and use of animals conformed to National Institutes of Health guidelines for humane animal care and use in research, and all protocols were approved by the institutional Animal Care and Use Committee. Mature male golden hamsters were housed in the university animal care facility on a 14L:10D cycle. Animals were killed with CO2, […]

20 Jan

Isoforms of the Mitochondrial Capsule Protein: INTRODUCTION

After exiting the testis, mammalian spermatozoa undergo morphological and biochemical changes as they travel through the epididymis. As a result of these modifications, spermatozoa acquire progressive motility and fertilizing potential; however, they are still fertilization incompetent. To achieve fertilizing capacity, spermatozoa require residence in the microenvironment of the female tract for a finite period of […]

25 Dec

Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome: DISCUSSION

Pig oocytes undergo GVBD in a similar time course required to activate the Cdc2 kinase. Following the gonadotropic stimulation, pig oocytes start to accumulate cy-clin B1 at 21 h, and Cdc2 kinase activity gradually increases around GVBD at 24 h and reaches a maximal level at the metaphase I at 27 h in our culture […]

23 Dec

Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome: RESULTS

Phosphorylation of Histone H3 Takes Place During Oocyte Maturation Phosphorylated histone H3 at Ser10 was detected in pig maturing oocytes using an antibody against histone H3 with a single phosphorylated Ser10. Histone H3 phosphorylation was not detected in any oocytes at the GV stage before culture (0%, 0/41 oocytes; Fig. 1A”). Phosphorylation of histone H3 […]

21 Dec

Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome: MATERIALS AND METHODS

Culture of Pig Oocytes Pig ovaries were obtained from prepubertal gilts at a local slaughterhouse. The ovaries were washed once with 0.2% (w/v) cetyltrimethylam-monium bromide and twice with Dulbecco phosphate-buffered saline (PBS) containing 0.1% (w/v) polyvinyl alcohol (PbS-PVA; Sigma Chemical Co., St. Louis, MO). Antral follicles 4-6 mm in diameter were dissected in PBS-PVA from […]

19 Dec

Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome: INTRODUCTION

The meiotic resumption of oocytes is controlled by protein kinase MPF (maturation-promoting factor or M-phase promoting factor), which is now also known as Cdc2 kinase. Cdc2 kinase is activated around the germinal vesicle breakdown (GVBD) of oocytes and has been thought to be the key regulator of such major morphological changes during oocyte maturation as […]

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