Archive for the 'Ovary' Category

17 Dec

Soluble Guanylyl Cyclase: DISCUSSION

The present study provides new information regarding the cellular localization and regulation of sGC a1 and p1 subunit levels in ovarian cells. Given the transient expression of sGC proteins in granulosa cells during follicle growth and the detection of these subunits in theca cells and corpora lutea, our findings suggest that sGC (and the associated […]

15 Dec

Soluble Guanylyl Cyclase: RESULTS

Expression of sGC a1 and ft] Subunits in Whole Ovary and Isolated Granulosa Cells Immunoblot analysis of sGC a! and p! subunit proteins was performed using protein extracts of whole ovaries and of isolated granulosa cells obtained from immature rats. When blots were incubated with primary antisera directed against the sGC a! subunit, an immunoreactive […]

13 Dec

Soluble Guanylyl Cyclase: MATERIALS AND METHODS

Hormones and Reagents Highly purified ovine FSH (oFSH-19-SIAFP; 94 X National Institutes of Health [NIH] FSH S1 U/mg; LH activity, 0.025 X NIH LH S1 U/mg) was obtained through the National Hormone and Pituitary Distribution Program (NIDDK, NIH). Androstenedione, eCG, and hCG were purchased from Sigma Chemical Co. (St. Louis, MO), and McCoy 5A medium […]

11 Dec

Soluble Guanylyl Cyclase: INTRODUCTION

The actions of FSH and LH on ovarian functions are mediated in large part through increased production of the second-messenger cAMP and subsequent activation of downstream signaling pathways. Although the importance of cAMP as a second messenger influencing the ovary is well recognized, other hormones and factors acting through diverse signaling pathways also modulate ovarian […]

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