Archive for the 'Oocyte' Category

03 Feb

Dynamic Changes in Meiotic Progression: DISCUSSION

Numerous studies have examined the effects of FSH, LH, and EGF on oocyte maturation, but very few have investigated their influence on the dynamics of meiotic progression in relation to cytoplasmic maturation. This may result from the lack of a suitable IVC system. Some of the terms used to describe effects on nuclear maturation are […]

01 Feb

Dynamic Changes in Meiotic Progression: RESULTS

Experiment 1 In the pig, MI is a relatively long component of the meiotic transition from GV to MII compared with other phases (the diakinesis of prophase I, prometaphase I, AI, and TI). Therefore, GV, MI, and MII were used as representative stages to indicate meiotic progression. As shown in Figures 1 and 2, more […]

30 Jan

Dynamic Changes in Meiotic Progression: MATERIALS AND METHODS

Culture Media All chemicals and reagents were from Sigma-Aldrich (Poole, U.K.) unless otherwise stated. The basic defined maturation medium (medium B) was Medium 199 containing Earle salts, 25 mM Hepes and sodium bicarbonate, 3 mM L-glutamine, 0.1% (w/v) BSA, 0.57 mM cysteine, 100 IU/ml of penicillin, and 0.1 mg/ml of streptomycin. The basal medium was […]

28 Jan

Dynamic Changes in Meiotic Progression: INTRODUCTION

Pig oocytes have been successfully matured and fertilized in vitro (IVF), and in vitro culture (IVC) conditions that support development to the blastocyst stage have been established. Recent work has focused on improving the culture conditions for in vitro maturation (IVM) to improve the efficiency of in vitro production (IVP) of embryos. In early studies […]

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