Archive for the 'Epithelial barrier' Category

22 Aug

Control the epithelial barrier: A pivotal first line of defense (Part 4)

Likewise activation of proteinase-activated receptor 1 on SCBN cells results in altered ZO-1 distribution and decreased barrier function that were prevented by pretreatment with inhibitors of caspase-3 or MLCK activation. This begs the question: is the increase in epithelial permeability observed following Giardia infection due to a parasite-derived proteinase? An intriguing possibility! Moreover, these observations […]

21 Aug

Control the epithelial barrier: A pivotal first line of defense (Part 3)

The idea that microbes could alter epithelial barrier function is not new nor is the observation that MLCK is a controller of paracellular permeability , so why is the report by Scott et al important? First, epithelial permeability is increased in human giardiasis and so the murine and in vitro models are directly relevant to […]

20 Aug

Control the epithelial barrier: A pivotal first line of defense (Part 2)

Juxtaposing murine in vivo and in vitro epithelial cell culture studies, Scott et al assessed if, and then how, infection with the protozoan parasite, Giardia spp, might affect epithelial permeability. Earlier work by this group had shown that the barrier function of human nontransformed small bowel-derived epithelial cell (SCBN) monolayers was perturbed by exposure to […]

19 Aug

Control the epithelial barrier: A pivotal first line of defense (Part 1)

Lumen-derived material gains access to the mucosa by permeating between adjacent epithelial cells (ie, paracellular pathway), by transcytosis across the apical and basolateral cell membranes (ie, transcellular pathway) or by exploiting breaks or erosions in the epithelium that may, for example, result from inflammation. Increased epithelial permeability (or decreased barrier function) has repeatedly been demonstrated […]

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