Archive for the 'Embryo' Category

02 Jan

Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos: DISCUSSION

All of the genes evaluated in this study are present in mRNA form in all pre-MZT embryo stages. This suggests that the early embryo could translate these mRNAs into proteins to use these factors during development. Of interest is the fact that the highest level of mRNA is found in the GV oocytes for all […]

31 Dec

Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos: RESULTS

Our results show that all the factors examined are present throughout the period studied (i.e., from GV oocytes to the blastocyst stage). The transcription factors examined were classified into five groups, based on their expression patterns throughout these developmental stages. The results have been presented in five different figures (Figs. 1 through 5) and significant […]

29 Dec

Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos: MATERIALS AND METHODS

Note that all chemicals were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise stated. Oocytes Recovery and Selection Bovine ovaries were collected at a commercial slaughterhouse and transported to the laboratory in a 0.9% NaCl aqueous solution containing antimycotic agent. Cumulus-oocyte complexes (COCs) from 3- to 6-mm follicles were collected with an 18-guage needle attached […]

27 Dec

Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos: INTRODUCTION

As observed in other species, the mRNA and protein content of the bovine oocyte supports the embryo’s first instances of life. It is believed that the mRNAs and proteins acquired by the oocyte during its growth and final maturation allow the zygote to go through the early stages of embryo development up until the moment […]

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