07 Feb

Aryl Phosphate Derivatives of Bromo-Methoxy-Azidothymidine: MATERIALS AND METHODS(14)

In Vivo Local Toxicity Studies

Thirty adult female CD-1 mice were randomly divided into two groups (A and B). Each group was further divided into three subgroups of 5 mice. Mice from group A were treated for 5 days and mice from group B for 20 days via daily intravaginal application of a cream base (Taro Pharmaceuticals) supplemented with 1% DMSO alone (control), 5% WHI-07 in 1% DMSO, or 5% N-9. After 5 and 20 days, respectively, mice were killed by cervical dislocation, and tissues from the genitourinary tract were fixed in 10% buffered formalin. buy flovent inhaler

To determine the degree of inflammation and membrane integrity of squamous epithelia, conventional paraffin-embedded sections (6-^m) were prepared and stained with hematoxylin and eosin and observed under X300 magnification with a Leica (Milton Keynes, Buckinghamshire, UK) microscope interfaced with an image analysis system. The images were captured using the ImagePro Plus program (Media Cybernetics, Silver Spring, MD) in conjunction with a 3CCd camera (DAGE-MTI Inc., Michigan City, KS), and images were transferred to Adobe Photoshop software for printout. The four stages of the estrous cycle were determined histologically.

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