Aryl Phosphate Derivatives of Bromo-Methoxy-Azidothymidine: DISCUSSION(3)
The human sperm-zona recognition and binding assays and the zona-free hamster egg binding and penetration assays are useful in predicting the in vivo fertilizing capacity of human sperm. We investigated the effects of dual-function AZT derivatives, WHI-05 and WHI-07, on the membrane fusogenic potential of sperm in the hamster egg penetration assay. Both these AZT derivatives that affected sperm motility also inhibited binding and fertilization of zona-free hamster eggs in a concentration-dependent manner. birth control yasmin
Furthermore, using a novel two-colored DNA-stained sperm-zona binding competition assay and confocal microscopy, we also demonstrated the concentration-dependent capacity of WHI-05 and WHI-07 treatment to inhibit binding of sperm to human zona as compared with that of control sperm using the same human zona. The use of cell-permeant DNA specific fluorescent dyes SYBR 14 and SYTO 17 to bind zona enhanced the specificity of the human sperm-zona binding test in comparison to conventional two-colored sperm-zona binding assays that employ nonspecific fluorescein- and rhodamine-labeled protein tags to detect fluorescent sperm binding to zona.